FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26511542
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTTCCAGTTTTCCACTTCAAA461210.17396573914863195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC145600.019.0819741
CGAGCAC195150.019.0081861
AGAATAG228550.018.9390621
CGGAGCT151850.018.8761671
GGCTCGA132550.018.7951771
CGGACGT124800.018.720211
AGCGTAT226350.018.6812921
GATGAAA135950.018.656171
GCATGGC239000.018.6553210
TACGGAT133550.018.4747121
CCGAATG152200.018.4609819
TCGCGAG82450.018.4497721
GAACCTA169950.018.4311731
ACGAGGA75950.018.4222371
GTCCACG201400.018.3954287
GTAAGAG142750.018.3949648
TCAGATG181700.018.3841691
CGAAGAG72000.018.333995
AAGTCTA135150.018.2892369
TAAGAGG126300.018.27249
Produced by FastQC (version 0.11.5)