FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27443994
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAAGG118700.018.5590931
ACTGATG147950.018.5481661
CGGACGT81000.018.4895741
TGACGGC118000.018.4487551
GGCGACT67250.018.4467351
GGACGAA55400.018.3566069
GGGAATG111400.018.3114261
GGAGCGA70150.018.2885889
CCGGTAG94200.018.2882611
GAGCAGA71850.018.254341
GGAAAGC88950.018.1754671
AAATGCC147750.018.1670061
AGATTGC168600.018.1099431
AGATGCA115500.018.0247673
AGGACGA56500.017.9992228
AACGCGA165000.017.9029410
CAATGGA82750.017.848178
CTCGAAA96500.017.831641
ACAGCCG82950.017.8257641
GATGCAA96950.017.7811074
Produced by FastQC (version 0.11.5)