FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28369502
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAAGG125500.018.6551821
TGACGGC116400.018.6358131
ACTGATG145650.018.5668411
GGAGCGA74450.018.5621599
CGGACGT95650.018.3812771
GGAAAGC87350.018.2959791
GGACGAA56500.018.2471649
AGATTGC168050.018.216571
GGCGACT67200.018.2011571
CCGGTAG102450.018.137421
AGGACGA56850.018.1347928
ACAGCCG119050.018.0613771
GAGCAGA72500.018.0155261
AAATGCC151500.017.9621261
GGGAATG112600.017.9502181
CCCAATC143650.017.9411621
AGAGCGA64700.017.8997121
GACCTGG108450.017.8071541
GACGACG72000.017.791069
AAGACCT105850.017.7626911
Produced by FastQC (version 0.11.5)