FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28277733
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAGA601100.019.7149149
CAGGGAG601650.019.7002148
GGGAGAG601300.019.69671210
ACAGGGA601950.019.6355767
AAGCGAG420450.019.5784199
GATCGCG350900.019.5738621
TACAGGG605750.019.5701756
GTACAGG605650.019.5420365
GGTGTAG693750.019.52660410
CCTAAGT662800.019.5091233
AGCCTAA657500.019.5033861
GCCTAAG662500.019.4912362
CTCAGAA457700.019.485481
ACGATGT422100.019.4744151
ATGGCGG284200.019.4615632
GGCGGTA284450.019.429884
AGTACAG609750.019.4171944
TGGCGGT285900.019.415353
CTGTAGG534550.019.413866
GATGAGC303950.019.41377810
Produced by FastQC (version 0.11.5)