FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25191584
Sequences flagged as poor quality0
Sequence length26
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGAGA537650.019.7259249
CAGGGAG538050.019.7242688
GGGAGAG537650.019.71104410
ACAGGGA539500.019.6342267
TACAGGG541650.019.5970236
AAGCGAG379000.019.587739
GATCGCG308550.019.5241761
GCCTAAG577250.019.5188062
ATGGCGG254000.019.502132
GGTGTAG603900.019.49760610
GTACAGG544450.019.4926415
AGCCTAA576100.019.4900341
GGCGGTA253950.019.4837284
ACGATGT382100.019.4620841
CTCAGAA398500.019.4467331
TGGCGGT255650.019.437893
CCTAAGT579750.019.4353983
CTGTAGG478900.019.433626
CAAGGCC301250.019.4162651
ATCGCGG305350.019.4159092
Produced by FastQC (version 0.11.5)