FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25191584
Sequences flagged as poor quality0
Sequence length124
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2385670.9470107159597427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1946650.7727382287671947No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1374660.5456822405450964No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA986730.39169033594711633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA659160.2616588143087787No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN359290.14262302838916363No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG288270.11443107348866988No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA287080.11395869350652979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1642200.0101.55211
AGCAGTG1695200.097.943452
GTGGTAT1888550.087.920296
GTATCAA1924700.086.5331959
GGTATCA1942200.085.489658
CAGTGGT1973150.084.0516364
TGGTATC1995500.083.1822747
GCAGTGG2026800.081.8113563
AGTGGTA2241450.074.170425
ATCAACG1857850.047.9716410-11
CAACGCA1860250.047.8217112-13
TACATGG1897350.046.24544522-23
GCAGAGT1950050.045.50671816-17
AGAGTAC1958600.045.38585718-19
TCAACGC1846200.045.00855312-13
GAGTACA1886900.043.96132320-21
AGTACAT2019550.043.9288220-21
GTACATG1901450.043.31781422-23
CGCAGAG1918900.043.24538816-17
CAGAGTA1970000.042.26395818-19
Produced by FastQC (version 0.11.5)