FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24682495
Sequences flagged as poor quality0
Sequence length124
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1424340.5770648388665732No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA497680.20163277658923864No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN350350.14194270068726844No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA321900.13041631326168607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT987500.088.699631
AGCAGTG1003400.086.939822
GTGGTAT1021800.085.32576
GTATCAA1070300.081.6138159
GGTATCA1095050.079.739248
TGGTATC1145750.076.18827
CAGTGGT1167300.074.826594
GCAGTGG1248000.069.945823
AGTGGTA1365000.063.977675
ATCAACG994700.047.35300410-11
CAACGCA997400.047.1689112-13
TCAACGC982150.044.46883812-13
TACATGG1049550.044.2232322-23
GCAGAGT1101300.042.6872616-17
GAGTACA1021250.042.53620-21
AGAGTAC1108800.042.27037418-19
GTACATG1039750.041.5900222-23
CGCAGAG1066150.041.02372716-17
AGTACAT1149900.040.583320-21
CAGAGTA1109000.039.39838418-19
Produced by FastQC (version 0.11.5)