FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25119871
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGA134100.019.0343691
CGGACTG146150.018.9432221
GACGGCT137100.018.8002471
ACTGAGT176800.018.7480811
AGGGATG127150.018.6667181
GCGCGAT130800.018.6428621
GATGGCG104350.018.6410562
CCGGTAG132550.018.6306481
CGGAGAG62700.018.5654493
CAGAGAG110200.018.5517921
TTGAACG111550.018.5335031
AGAGAAC102450.018.5060048
TTCGAAG152000.018.4971081
AGGACGA75400.018.4609558
GGTGAGG106250.018.4371017
GAGGGTG81650.018.4074764
CACTGCG136550.018.40293510
GTCGGGT98450.018.3987921
AACGGAG82700.018.3936122
ATAGGAG76300.018.38736210
Produced by FastQC (version 0.11.5)