FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25119871
Sequences flagged as poor quality0
Sequence length124
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1215610.48392366346148835No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1189170.47339813170218903No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAAAAAAAAAAAAAAA969740.38604497610676425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG544750.21686019008616725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA424540.16900564497325643No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC371260.14779534496813299No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN356770.14202700324376666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1106000.092.475311
GGTATCA1179700.086.3733448
GTGGTAT1181550.086.2163856
GTATCAA1188350.085.9431469
TGGTATC1192100.085.479337
CAGTGGT1277550.079.780414
AGCAGTG1456100.070.392812
GCAGTGG1793500.057.2737433
AGTGGTA1876700.054.459735
ATCAACG1119200.048.74135210-11
CAACGCA1121750.048.5558312-13
TCAACGC1113150.045.92630812-13
AGAGTAC1197950.045.3674318-19
TACATGG1192250.045.1279222-23
GCAGAGT1240400.043.82020616-17
AGTACAT1239350.043.72471620-21
CGCAGAG1174550.043.51957716-17
GAGTACA1175650.043.3314820-21
GTACATG1181300.042.89659522-23
TATCAAC1219650.042.0698310-11
Produced by FastQC (version 0.11.5)