FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30731529
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG245850.019.06767710
ATCGAGT244500.018.8688451
GATCGCG138400.018.7205751
CCGTGGA127900.018.6769141
ACTCTCG296400.018.656476
CCGGGAT137550.018.5961691
TCTCGGA252500.018.593228
TGGGCGT120500.018.5033911
TCGCGAG139150.018.4039171
GGGCGTC73050.018.3844072
CGGACTG117200.018.3242131
CGAGCAC115550.018.3173921
GGTGTCG114900.018.28464110
GCGAGTC101250.018.270873
GTGCTAG138800.018.2556618
CTAAGAC135100.018.2148821
CTCTCGG257400.018.1965377
ATGCGAT109750.018.182091
ACGGAGA94950.018.1493641
CACGGTA212200.018.1141688
Produced by FastQC (version 0.11.5)