FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30123135
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGACG230650.018.99356310
CCGTGGA124400.018.7525331
CCGGGAT135650.018.6722091
ATCGAGT231000.018.664531
GATCGCG132550.018.6258961
ACTCTCG277000.018.5809356
TCTCGGA236850.018.4794838
TCGCGAG135850.018.4606271
TGGGCGT108950.018.2808251
CGAGCAC114350.018.2311171
CTAAGAC127800.018.222331
GGGCGTC66400.018.1779062
GCGAGTC103100.018.166693
GGTGTCG106600.018.16079310
CTCGGAC241600.018.1451389
CACGGTA207450.018.0953718
CTCTCGG241300.018.0558077
CGGACTG102450.018.0541361
CTACTCT258950.018.0380364
ATGCGAT111250.017.974821
Produced by FastQC (version 0.11.5)