FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25996574
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTC214450.019.281234
TAAGAGA233550.019.1676941
GCTGCGA192950.019.1578541
ACGGAGA152350.019.1427671
TTCGGGG214400.019.1419532
AGAGCGA116500.019.127091
GGGATGA159550.019.1251581
CTAATAG401300.019.1123833
TCTAATA400750.019.1093312
CAGCGAG102850.019.086212
TCGGGGT218900.019.0036143
CGGGAAG105950.018.904653
CGGAGAG89700.018.896482
CAGGAGC152800.018.8864697
GAGCGAA93800.018.8807562
ACCAGTA426450.018.877021
GGGGTCT219000.018.871475
CTGTAGG185650.018.8304984
CTAACGT291700.018.82349810
TCTAACG292000.018.82119
Produced by FastQC (version 0.11.5)