FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004996628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004996628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24753238
Sequences flagged as poor quality0
Sequence length26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGCG210650.019.0931761
GCGTATA232550.019.0100542
GTATAGC233500.018.9413914
GATCGCG138250.018.9293691
AGCGTAT245100.018.9146211
TATAGCA233500.018.8985655
TGACGGC125850.018.8397851
CTCGTAC295800.018.7727171
GCACGCC257300.018.7166299
AGCGGAT141950.018.6324658
TAAGAGA138800.018.5733851
CAGCGAC99950.018.5191571
GAATGAA136300.018.5178721
ACGAGAG109600.018.5028369
TAAGACG108800.018.491762
CACGAGA109750.018.477518
CTCGAGG113000.018.4335271
GAGCGGA54550.018.422737
TCGGTAA208050.018.4041271
GCGGGTC96000.018.3741192
Produced by FastQC (version 0.11.5)