FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38645590
Sequences flagged as poor quality0
Sequence length41
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG600350.155347608873354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGT34250.013.84871229
ACGAGTG36950.012.88416430
CCGACGA40300.012.3774927
TATACTG41450.012.2846835
CGAGTGT42200.011.82011931
CGACGAG42300.011.75087428
TGCCGAC45800.010.92921125
TAGACTG56850.010.5882335
TACACTG66850.010.0775485
TCTATAC35500.09.9557683
GTGTAGG79450.09.9549721
GTATAGG38850.09.953981
CTAGACT57750.09.9076094
GTACTAG22800.09.82361
TAGACAG75900.09.5906295
GTATAGA39600.09.5887081
TAGGACC46700.09.5167774
TAGACCG21350.09.5072665
CTAGGAC79050.09.4731323
CTAGTAC22450.09.4301973
Produced by FastQC (version 0.11.5)