FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34321029
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32050.020.7781945
TCTCGTA32000.019.8263444
ATCTCGT47550.013.57917843
CGTCTGA56250.012.63781217
ACGTCTG70350.010.04090516
ACACGTC74800.09.44355314
CACGTCT78350.09.04438215
GATCTGA94750.07.883320337
AGTCACC98250.07.76316829
TCTGATC96400.07.374725339
CACACGT100700.07.327430213
TCTGAAC111700.07.02877419
AGATCTG111700.06.70719336
CAGTCAC111300.06.691220328
CCAGTCA115750.06.550614427
GTCTGAA118250.06.52529718
GTCACCA120500.06.516437530
CAGATCT117200.06.50761435
ACCAGAT118550.06.41451133
GCACACG117100.06.28200412
Produced by FastQC (version 0.11.5)