FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42266053
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTACTATCTCGTAT829240.19619527756708205TruSeq Adapter, Index 12 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT117700.035.5406345
TCTCGTA121850.033.29685244
TATCTCG129350.031.4711142
ATCTCGT145650.028.18043343
GTACTAT150350.027.29969638
TACTATC158450.025.91841739
TGTACTA167700.025.36020537
CTTGTAC170750.024.97309535
AGTCACC189300.022.76283329
ACTATCT183150.022.5703640
CCTTGTA192400.022.38507334
GTCACCT203250.021.2452530
TTGTACT203300.021.22911536
CAGTCAC204750.021.16594728
CTATCTC195700.021.15742941
CCAGTCA209700.020.6020227
ACCTTGT228150.019.08445233
TCCAGTC235450.018.51117126
CTCCAGT238550.018.22345725
CGTCTGA238800.018.063517
Produced by FastQC (version 0.11.5)