FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44232951
Sequences flagged as poor quality0
Sequence length41
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG52700.012.4856035
TCTATAC41300.011.7376773
GTATAGA41600.010.9402121
CTAGTAC26250.010.9336953
TAGACTG60150.010.6482385
GTGTAGG84800.010.5067321
GTCTAGA48500.010.394331
ATACTGT67500.010.3704216
TACACTG73900.09.9931025
TAGACCG26600.09.9340795
TACACCG34100.09.8532425
TAGACAG75650.09.5306075
CTAGGAC100900.09.487433
CTTAGAC46300.09.4873553
TCTAGAC52800.09.4794823
GTATAAG35400.09.4444321
CTAGCCT91450.09.4149424
TAGGACC60050.09.3838084
GTATAGG45500.09.271531
TAGGACA55650.09.2137984
Produced by FastQC (version 0.11.5)