FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31923913
Sequences flagged as poor quality0
Sequence length41
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTA18750.014.83956126
CGTGCGA30000.014.64201310
TCGTTGT29750.014.52936734
CATCGTT30200.014.31275832
TCGTTCG21000.013.41637423
CGAGAAT32250.013.24027114
TATACCG24700.013.1078646
TTGCGAT18550.012.9244716
TATACTG39950.012.8353395
GTATAGA50550.012.7400631
TTTAGCG23200.012.5211826
GTACAAG38350.012.00149251
CGGTAAG23750.011.86304828
TATCGGT23600.011.56754524
TCCTATA46400.011.4654652
GCGAAGT21550.011.44999232
TCCGTAT25050.011.31733113
TCGTTTA27100.011.17146632
ATCGGTT25200.011.0413425
GTATCGG24950.011.011991514
Produced by FastQC (version 0.11.5)