FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32255913
Sequences flagged as poor quality0
Sequence length41
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC34500.012.1736943
TATACTG32800.011.7382135
TCGTTGT19150.010.87459734
GTATAGA39500.010.6773381
TTATACA68550.010.0593435
CTAGCCT76400.09.8039614
CGTGCGA29050.09.7593310
GTATAGG47650.09.6958081
GTACTAG16250.09.6924191
TATACCG18350.09.6324866
CTAGTAC16550.09.6221843
CTTAGAC29450.09.5668913
TAGACCG14750.09.4918555
CCTATAC53050.09.3023783
GTACAAG35200.09.2472651
TTCGGTA13850.09.22366126
GTATTAG64900.09.1950211
TTAGGAC43300.09.093373
TCTACAC59400.09.0739123
GTGTAGG87650.08.9847011
Produced by FastQC (version 0.11.5)