FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32255913
Sequences flagged as poor quality0
Sequence length41
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA35000.011.8476611
TATACTG30600.011.3796215
TCTATAC36950.010.8891433
CGTGCGA30550.010.88299810
TATACCG17850.010.489416
TCGTTGT18150.010.41423634
TTATACA68050.010.31327335
GTACAAG33700.09.9683591
CTAGCCT77100.09.8718224
GCTATAC19700.09.6792243
GTATTAG63500.09.5059981
CGAGAAT28000.09.43774514
ATACTGT46700.09.2551966
TAGACCG15550.09.2274055
GTATAGG45850.09.1584981
GTGTAGG84400.09.0592311
GTCTAAG29450.09.0304761
CATCGTT21650.08.97313132
CTACACT71650.08.9377324
TTCGGTA15200.08.8657626
Produced by FastQC (version 0.11.5)