FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36736649
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC898870.24467936637334559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC107700.028.58535628
AGACCGA109050.028.29591431
GACCGAG113100.027.23857132
CCCACGA115750.026.52268625
GAGACCG117900.026.19965630
ACCGAGG119850.025.7188133
TCTCCGA125300.024.6923817
CCACGAG128600.023.9390326
TCCGAGC131250.023.62451219
CGAGCCC132450.023.28139521
ATCTCCG133350.023.20147916
CACGAGA138700.022.36745627
GCCCACG142150.021.63272724
CCGAGCC150900.020.72792620
CGAGACC161450.019.4271329
CTCCGAG170450.018.2714718
CCGAGGC177400.017.89321134
ACATCTC197550.015.87702314
TACACAT201350.015.610558511
TATACAC203150.015.4552759
Produced by FastQC (version 0.11.5)