FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36736649
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGA872360.23746313932988283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG484350.13184381623920025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA98600.030.44013427
CGACGAG99000.030.43818528
GACGAGT101250.029.79620429
CGAGTGT103250.029.31824731
TGCCGAC103100.029.14432725
GCCGACG108150.027.81578826
ACGAGTG111400.027.17325830
TGACGCT112300.027.06209419
CTGCCGA113200.026.70881524
TCTGACG115550.026.30315417
ACGCTGC119300.025.40310921
GACGCTG140750.021.8093320
CTGACGC140050.021.67578918
CGCTGCC146400.020.86199222
GCTGCCG148000.020.77369723
ATCTGAC148250.020.70884116
GAGTGTA163000.018.7480832
AGTGTAG167200.018.51981233
GTGTAGA169350.018.0941934
CATCTGA171950.017.89560715
Produced by FastQC (version 0.11.5)