FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91951827
Sequences flagged as poor quality0
Sequence length41
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG88600.011.6917955
TCTATAC73600.010.9590243
GTATAGA80000.010.6521471
TAGACCG56050.010.3334555
GTATAAG68200.09.9550981
TAGACTG126700.09.7503745
GACGAGT70200.09.62366529
GTATTAG110700.09.4526091
TACACTG158850.09.4513385
GTGTAGG185800.09.4084471
TACACCG69800.09.4008925
ATACTGT121800.09.3378486
CTATACT125100.09.2871554
CCTATAC100100.09.263833
TAGACAG146900.09.2553415
CTAGTAC49850.09.1957033
ACACCGT71600.09.0421016
GTATAGG92900.09.0411511
GTACTAG47250.08.9991731
TACACAG180200.08.9530155
Produced by FastQC (version 0.11.5)