FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49859252
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACACCACT1508920.30263590797551476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC179600.029.02352528
CGAGACA181600.028.68515829
CCCACGA190950.027.3159925
TCTCCGA206350.025.30610317
CCACGAG215500.024.27517326
TCCGAGC216100.024.18796519
ATCTCCG219800.023.76516216
CGAGCCC220350.023.76160621
CACGAGA226300.023.25926227
GCCCACG234150.022.46451224
GACACCA235100.022.45303332
CCGAGCC252650.020.90417920
GAGACAC264700.019.96880130
AGACACC271050.019.550431
CTCCGAG274750.019.10504718
TACACAT295950.017.80041311
ACATCTC302800.017.40253814
TATACAC305200.017.3276849
CATCTCC312850.016.90333615
GAGCCCA330500.016.31040222
Produced by FastQC (version 0.11.5)