FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37938381
Sequences flagged as poor quality0
Sequence length41
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG40350.012.9675755
TCTATAC29250.011.06876853
TATACAC78250.010.98075835
CTAGGAC67150.010.4769163
GTATAGA29550.010.2482631
GTGTAGG65850.010.2079161
TAGACTG50900.010.1078935
TAGACCG24550.010.0507565
GTCCTAG61200.09.9538141
CTACACT68700.09.8581034
CTATACT38550.09.8054724
TACACTG65050.09.7385075
TAGGACC48000.09.5885664
TCTACAC51250.09.5271673
TAGGACA43350.09.486764
TTATACA92450.09.48344135
ATACTGT57700.09.46286
CCTACAC64700.09.2236663
TAGACAG67350.09.0941385
GTCTAAG43350.09.0856311
Produced by FastQC (version 0.11.5)