FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37938381
Sequences flagged as poor quality0
Sequence length41
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG589700.1554362586004922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC73950.011.07611635
TATACTG39350.010.9394435
TAGACAG62700.010.7168835
TACACTG62150.010.2486125
TAGACCG25450.010.1760395
CTACACT66900.010.06974
GTGTAGG64900.09.7063971
TAGACTG49100.09.693765
CCTACAC67100.09.67394353
TTATACA90750.09.64280135
TACACCG26350.09.6292445
TCTAGAC41550.09.4746613
TCTATAC26550.09.4237483
GTCTAGA48150.09.4124721
GTATAGA28700.09.3894261
GTTCTAG56550.09.3448981
GTCTAAG43100.09.2567351
CTAGGAC65750.09.1540823
GTATAGG28700.09.1455451
ACCTGTC165800.09.09918635
Produced by FastQC (version 0.11.5)