FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36448449
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT30200.020.9266445
TCTCGTA31150.019.78318644
TATCTCG33200.018.69732542
CCGATGT41100.015.75993334
ACCGATG43500.014.838716533
CACCGAT46500.014.26827932
ATCTCGT44800.014.00658643
CGATGTT51800.012.93868635
GTCACCG55900.011.74819630
TCACCGA57150.011.33379131
CGTCTGA72850.09.13817117
ACGTCTG90750.07.385074616
AGTCACC99900.07.13650529
GTTTATC92000.07.113967439
CACGTCT105100.06.54786615
ACACGTC108450.06.179976545
GCACACG171550.06.135575345
TCTGAAC120100.06.086077719
CCAGTCA119400.06.0274727
CAGTCAC121550.05.883841528
Produced by FastQC (version 0.11.5)