FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47974769
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42300.022.49791745
TCTCGTA46550.019.4289244
TATCTCG53500.016.94699342
ATCTCGT65800.014.22356243
CGTCTGA87650.010.90801717
ACGTCTG106650.09.15449816
GGTATCT106950.08.961143540
GTATCTC109250.08.71087341
AGTGGTA125100.08.32669737
GTGGTAT118800.08.31375438
GTCACAC121700.08.20845430
TGGTATC120800.08.17584439
ACACGTC121550.08.069324514
CACGTCT125200.07.74423615
CAGTCAC151700.06.8076428
CCAGTCA155900.06.782990527
TCTGAAC154000.06.66120719
CACACGT154900.06.23033613
GTCTGAA175450.06.193021318
TCCAGTC178900.06.12474626
Produced by FastQC (version 0.11.5)