FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33467825
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC29200.028.64322928
GACGTGT30600.027.3353732
CCCACGA33850.025.15953325
AGACGTG34950.023.98096831
TTATACA169950.020.62815734
CGAGCCC43150.019.69748321
GCGAAGT25050.018.46045532
CGTGCGA32800.017.67319510
CGAGAAT35100.016.3704914
GAGACGT51450.016.32273730
CCGTGCG38350.016.31219
TCGTTGT38350.016.22589534
CATCGTT38900.015.99604832
GCGAGAA37450.015.75168713
TTGCGAT22700.015.57690116
ACGTGTG56800.014.90630633
TTCGGTA24950.014.85314926
TCTCCGA57450.014.67695317
TCGTTCG25250.014.67647823
TCCGAGC59150.014.65725119
Produced by FastQC (version 0.11.5)