FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33467825
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG337070.10071464159980517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG28100.029.03224228
CCGACGA28700.028.36611727
GACGAGT29250.028.12369229
CGAGTGT29150.027.98694431
TGCCGAC30500.026.96999225
GCCGACG32950.024.96538426
TGACGCT34550.023.90923719
CTGCCGA35100.023.72548724
ACGCTGC38250.021.81912221
TTATACA167750.020.51525734
TCTGACG45750.018.46646117
CGTGCGA31650.017.7856410
GCGAAGT26100.017.3865432
CGCTGCC50250.017.01330222
TTCGGTA24150.016.96087326
TCGTTCG24400.016.78666523
CGAGAAT34900.016.51489414
CCGTGCG38000.016.4706219
GCTGCCG52900.016.32094423
ACGAGTG50700.016.2586330
Produced by FastQC (version 0.11.5)