FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35820077
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTACG1484310.41437934374066254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC166850.030.13825828
CGCTACG169100.029.5896334
CCCACGA175900.028.5961225
GACGCTA175750.028.5562132
TCTCCGA185600.027.10228517
ACGCTAC188550.026.77151533
TCCGAGC188350.026.73320819
CGAGCCC188250.026.67453621
CGAGACG190150.026.49902729
CCACGAG189850.026.42386426
GAGACGC192550.026.24848730
ATCTCCG192350.026.14218516
GCCCACG199900.025.21368224
CACGAGA204200.024.85838927
CCGAGCC206500.024.51473620
AGACGCT213750.023.70169431
CTCCGAG232050.021.76518818
ACATCTC250850.020.26981414
TACACAT251700.020.18711911
CATCTCC258450.019.83134315
Produced by FastQC (version 0.11.5)