FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35820077
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGA1441320.40237769449797667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGA158000.031.28079827
CGACGAG159900.030.90906128
GACGAGT161400.030.7172629
TGCCGAC161300.030.66130425
CGAGTGT162250.030.3888231
GCCGACG166100.029.86850226
TGACGCT169050.029.30903419
CTGCCGA169600.029.24053824
ACGAGTG171800.028.7688630
TCTGACG173050.028.6541717
ACGCTGC175750.028.24957321
GACGCTG197000.025.31422820
CTGACGC200050.024.82712618
CGCTGCC203000.024.64830622
ATCTGAC203600.024.47244816
GCTGCCG205500.024.31485723
CATCTGA218850.022.85447915
GAGTGTA220150.022.49634632
GTGTAGA222050.022.4169734
AGTGTAG224200.022.31579433
Produced by FastQC (version 0.11.5)