FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36589934
Sequences flagged as poor quality0
Sequence length41
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG54450.011.8277315
TCTATAC45900.011.3998243
GTATAGA48950.010.4396051
ATACTGT71750.010.4393486
TATACCG18300.010.2325576
TAGACCG16800.010.104485
TAGGACC41000.010.0307854
TAGACAG73050.09.8223445
GTATTAG75400.09.7019451
GTATAGG52750.09.4553211
GTATAAG42800.09.281881
TCGTTGT19500.09.24350934
CTAGTAC21050.09.2280623
CGTGCGA26750.09.15906210
GTGTAGG86650.09.0684191
TACACTG72300.09.0528395
CTAGCCT85800.08.9746254
GTCTAAG37600.08.9364961
TAGACTG60950.08.9297275
GTATATA109150.08.8665621
Produced by FastQC (version 0.11.5)