FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36589934
Sequences flagged as poor quality0
Sequence length41
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG55800.012.5120725
GTATAGA47450.011.5418671
ATACTGT73600.010.4609566
TAGACCG17900.010.4597495
CTAGTAC20900.010.2118053
TCTATAC48800.010.0375393
CGTGCGA27200.09.77847510
TACACTG69900.09.6627475
GTATTAG78100.09.1630541
CTACACT84200.09.143284
GTACTAG21250.09.0573621
TAGACAG72700.09.0499035
GTATATA115600.09.0058991
CTAGCCT82250.08.9984124
TAGACTG59600.08.9545565
GTATAGG56200.08.8731231
GTGTAGG86400.08.7688391
TAATACG18200.08.7484564
GTGTAAG75900.08.7370621
TACACAG71150.08.73059755
Produced by FastQC (version 0.11.5)