FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35743184
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC1325090.3707252269411701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC155500.029.10138128
CCCACGA165200.027.48437125
ACTAAGG169200.026.9393133
GACTAAG169250.026.86043532
TCTCCGA173000.026.27551717
CGAGACT175950.025.94165229
TCCGAGC178350.025.46790519
CCACGAG178550.025.43916326
CGAGCCC179300.025.33232721
ATCTCCG181750.025.03869616
AGACTAA183400.024.92622831
CACGAGA186950.024.4234727
CTAAGGC188750.024.35645134
GCCCACG190500.023.87869824
CCGAGCC197550.023.16424620
GAGACTA201300.022.86079830
CTCCGAG218850.020.91050118
TACACAT233100.019.7419411
TATACAC234500.019.4352689
ACATCTC237750.019.21332614
Produced by FastQC (version 0.11.5)