FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35743184
Sequences flagged as poor quality0
Sequence length40
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGA1287410.3601833569163844No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG809360.22643757758122499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGT147900.030.09660531
GACGAGT150650.029.55824929
CCGACGA151850.029.22327627
CGACGAG154700.028.76188728
TGCCGAC156550.028.49751525
TGACGCT159400.028.03354319
ACGAGTG160750.027.65891530
CTGCCGA162300.027.49794424
TCTGACG162800.027.39741117
GCCGACG164800.026.96853626
ACGCTGC169100.026.49575821
GACGCTG189350.023.7246120
CTGACGC191250.023.38249618
CGCTGCC193450.023.30011422
ATCTGAC193300.023.2341716
GCTGCCG200700.022.49989123
AGTGTAG205900.021.92183733
GAGTGTA204950.021.85944732
GTGTAGA207700.021.78122134
CATCTGA208900.021.5987315
Produced by FastQC (version 0.11.5)