FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20432613
Sequences flagged as poor quality0
Sequence length41
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG18500.010.9716065
TCTATAC16350.010.8064583
GTATAGA15700.010.0297881
TAGACCG10450.09.8791525
GTTCTAG25550.09.8609891
TAGACAG32150.09.7966035
CTATACT24500.09.6409484
TACACTG31250.09.63085
TAGGACA24500.09.4981194
CTAGTAC11000.09.3829443
CTAGACT27900.09.2813174
CTAATAC25750.09.239353
ACACCGT15950.09.2153336
CTAGCCT43400.09.1917294
TAGACTG28500.09.1479845
CCTATAC21200.09.076883
TCTAGAC21300.09.03426553
TATACAG17300.09.0017695
GTCTAGG36800.08.9859271
TACACCG15900.08.9139835
Produced by FastQC (version 0.11.5)