FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44389176
Sequences flagged as poor quality0
Sequence length40
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750690.16911555195347622No Hit
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGA527590.1188555516326773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAGT69250.026.7275329
CCGACGA70100.026.3787827
CGAGTGT71600.025.89786131
CGACGAG72250.025.61686928
TGCCGAC74000.025.14977325
ACGAGTG78450.023.65812930
TGACGCT81850.022.89322319
TCTGACG88500.021.09838117
CTGCCGA89700.020.8815324
GCCGACG92250.020.37687126
ACGCTGC96050.019.5034421
TTATACA247100.015.99178734
GACGCTG120000.015.77082920
CTGACGC128050.014.74025718
CATCTGA131250.014.62803915
ATCTGAC137300.013.822453516
GAGTGTA139400.013.35068532
AGTGTAG145500.013.17632733
GTGTAGA147150.013.04008634
CGTGCGA28650.012.93671410
Produced by FastQC (version 0.11.5)