FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81676639
Sequences flagged as poor quality0
Sequence length41
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG95950.012.6744655
GTATAGA83300.011.2032541
TCTATAC86150.010.4199913
ATACTGT125150.010.3045086
TAGACCG46600.010.025715
GTATTAG132050.09.6395771
TAGACAG131750.09.1773495
GTATAGG105500.09.0947341
GTATATA173800.08.9459221
TACACTG130750.08.9129695
CTACACT173600.08.8897394
GTGTAGG183900.08.8640021
GCTATAC48350.08.7945793
GACGAGT59500.08.79427729
TAGACTG118800.08.7195715
TACACCG75300.08.6212215
CTAGTAC44150.08.6007093
GTCTAAG75950.08.5527941
CGTGCGA75300.08.55153810
CTAGCCT176250.08.5376724
Produced by FastQC (version 0.11.5)