FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30558738
Sequences flagged as poor quality0
Sequence length37
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC33000.018.3293814
CGTCTGA36550.016.6813117
ACGTCTG41650.014.67717416
ATCGGAA46200.014.2685063
GATCGGA48750.013.7749682
CACGTCT45200.013.66679415
CACACGT48400.012.63025413
TCGGAAG53450.012.3905994
CGGAAGA54200.012.3887115
AGATCGG56550.011.9059811
GCACACG59850.010.61712212
TCTGAAC75900.08.72711719
GTCACAT74500.08.65323330
TCACATC79500.08.47585431
GTCTGAA79450.08.19991618
AGCACAC83500.07.95687711
CAGTCAC87650.07.862846428
AGAGCAC85650.07.7676869
CCAGTCA86450.07.687896727
CTGAACT92000.07.3498820
Produced by FastQC (version 0.11.5)