FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41433490
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT19750.014.46403645
TCTCGTA22950.012.74129344
ATCTCGT32700.09.01107143
CGTCTGA51300.05.96253517
ATCGGAA281350.05.79620845
CGGAAGA235100.05.66398345
ACACGTC64700.05.49295145
GATCGGA317900.05.490653545
TCGGAAG257300.05.47252145
AGATCGG365900.05.13306745
Produced by FastQC (version 0.11.5)