FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45234537
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT52100.028.58071745
TATCTCG55700.026.32965942
TCTCGTA56400.025.9231144
ATCTCGT65150.022.54507343
TACTATC86600.017.428939
GTACTAT89100.016.73803738
CTTGTAC108100.014.39957235
TGTACTA110600.014.13509837
CGTCTGA116600.013.27078617
ACTATCT121300.012.47992240
CCTTGTA134950.011.78371134
ACGTCTG139450.011.17690316
TTGTACT143550.011.14129336
CTATCTC147300.010.59776841
AGTCACC162050.010.13264529
ACCTTGT165600.09.901628533
ACACGTC156000.09.89022714
CACGTCT161550.09.73143715
GTCACCT177200.09.39329530
CCAGTCA176300.09.39024527
Produced by FastQC (version 0.11.5)