FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292436
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT657910.3596623216284589TruSeq Adapter, Index 8 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA81500.027.1470173
CGTCTGA79300.026.95828617
GATCGGA82950.026.804342
ACACGTC80200.026.63542714
AGATCGG85550.025.9454211
ACGTCTG82800.025.81811116
TCGGAAG85400.025.744244
CGGAAGA86200.025.514625
CACGTCT85900.024.90433915
CACACGT89350.024.02915213
GCACACG92850.023.52377312
GTCACAC101400.020.91699830
TCTGAAC103800.020.75957919
AGCACAC108300.020.32041711
GTCTGAA107700.020.20921118
AGAGCAC108600.020.2075659
CCAGTCA106900.020.0146827
GAGCACA111300.019.85679810
GAAGAGC113450.019.5079577
CAGTCAC111500.019.21680628
Produced by FastQC (version 0.11.5)