FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24007493
Sequences flagged as poor quality0
Sequence length37
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT398570.16601900081778637TruSeq Adapter, Index 9 (100% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA50000.027.9352252
AGATCGG51450.027.3957421
ATCGGAA50700.027.3349253
CGTCTGA47600.026.9302817
GTCACGA49850.026.43530830
TCGGAAG52550.026.255864
ACACGTC49150.026.08033214
TCACGAT52000.025.28309631
AGTCACG52550.024.95988729
CGGAAGA55800.024.643155
ACGTCTG52400.024.46275516
CACGTCT54650.023.48469715
GCACACG58850.022.26805712
CACACGT60500.021.37158813
AGAGCAC86850.016.0975869
GAAGAGC86600.016.0198357
TCTGAAC86750.015.92406919
AGCACAC87350.015.45778711
CCAGTCA88450.015.22954227
GTCTGAA91250.015.152618
Produced by FastQC (version 0.11.5)