FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57865208
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51800.026.27078445
TCTCGTA53400.024.6833344
TATCTCG57800.022.49300242
ATCTCGT68900.019.16312243
CACTAGC110100.013.19766532
GTCACTA119950.012.02009130
ACTAGCT126750.011.658771533
CGTCTGA120800.011.61779817
TCACTAG132900.010.95034531
GCTTGTA136800.010.67145437
CTAGCTT145050.010.23422134
GTATCTC143100.010.02859441
TAGCTTG148150.09.79317435
ACGTCTG150400.09.43599416
CTTGTAT159000.08.95510938
AGCTTGT165350.08.82888636
ACACGTC163850.08.62023814
TTGTATC163150.08.5894439
CACGTCT174950.08.17615915
CCAGTCA211400.07.182081727
Produced by FastQC (version 0.11.5)