FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35308926
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT30950.025.65472445
ACGGCTA31950.025.06228833
TCTCGTA31200.024.51193644
CGGCTAC41250.019.4659734
ATCTCGT42100.018.37932843
AGTCACG47750.017.19378729
GTCACGG48800.016.86995530
TCACGGC57800.014.20416931
CACGGCT60950.013.69169432
CGTCTGA76200.010.77322317
GGCTACA95250.09.16297735
ACGTCTG94950.08.93005616
GCTACAG108300.08.24578336
GATCTCG110250.07.997619642
CACGTCT110550.07.77163515
AGATCTC111850.07.58162641
ACACGTC108650.07.534933614
ACAGATC138200.06.884865339
CCAGTCA130550.06.788491227
CAGTCAC132450.06.775993328
Produced by FastQC (version 0.11.5)