FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25123921
Sequences flagged as poor quality0
Sequence length37
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGC905810.3605368763896368TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA111350.027.60649517
ACACGTC111700.027.4620214
ATCGGAA124800.026.9153753
ACGTCTG114400.026.85599516
GATCGGA125800.026.8505652
AGTCACG119550.026.38153629
CACGTCT117850.026.15156615
AGATCGG130700.026.0169891
GTCACGG123500.025.67451930
TCGGAAG132250.025.294484
CGGAAGA133750.024.9301325
TCACGGC127150.024.88924231
CACACGT124250.024.72696913
GCACACG137450.023.00664312
TCTGAAC148100.021.77344919
GTCTGAA151600.021.39626918
AGAGCAC160000.020.6759999
AGCACAC156500.020.36021411
CCAGTCA157000.020.29395927
CAGTCAC159650.020.0148928
Produced by FastQC (version 0.11.5)