FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19140240
Sequences flagged as poor quality0
Sequence length37
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC19900.019.06579614
GATCGGA20000.018.9180432
ATCGGAA21200.018.065983
CGTCTGA21400.017.51350217
AGATCGG23450.016.2730031
TCGGAAG24150.015.9882464
ACGTCTG24650.015.51815616
CGGAAGA26650.014.3377055
CACGTCT27100.014.17215
CACACGT29250.012.97125813
GCACACG32900.011.62633912
TCTGAAC45250.08.79601519
GTCTGAA47800.08.29437218
AGAGCAC49450.08.1975289
GAAGAGC51100.08.1760937
AGCACAC50550.07.803704311
CCAGTCA49600.07.744544527
CAGTCAC51750.07.66227628
AGTCACT54150.07.55148729
GAGCACA54450.07.33067510
Produced by FastQC (version 0.11.5)