FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46114016
Sequences flagged as poor quality0
Sequence length40
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1145290.24836049846536898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA313250.021.85467334
GACGAGT45700.021.19870229
CCGACGA50750.019.15602527
CGACGAG53400.018.55528328
CGAGTGT52700.018.51189831
ACGAGTG53200.018.14624830
TGCCGAC57150.016.98120925
CTTATAC435800.015.73618433
TGACGCT65450.015.12000419
TCTGACG70750.014.10889817
TCTTATA541900.012.74939632
CTGCCGA80800.012.34790824
ACGCTGC93250.011.04722121
TATACTG66100.011.0074375
GCCGACG94050.010.78852126
CTCTTAT639650.010.75061131
ATCTGAC99050.010.215219516
GAGTGTA98550.09.98550832
GTATAGA57200.09.6006951
TCTCTTA734400.09.53023930
Produced by FastQC (version 0.11.5)