FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004998641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004998641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36776629
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTGTATCTCGTAT373460.10154818702932235TruSeq Adapter, Index 10 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59700.030.22329945
TCTCGTA60500.028.70805544
TATCTCG68700.025.41253742
ATCTCGT81300.021.7507943
CGTCTGA86350.021.54518117
CACTAGC88850.020.89039432
GTCACTA94100.019.70167230
ACGTCTG98050.019.11184716
ACTAGCT98050.018.8384733
TCACTAG101150.018.5261931
ACACGTC103150.017.99243514
CACGTCT105250.017.95404815
GTATCTC109050.016.33966341
CTAGCTT114900.016.13456734
GCTTGTA118600.015.47951537
TAGCTTG123800.015.08372935
CACACGT131450.014.32419113
CAGTCAC134400.014.26283228
CTTGTAT129200.013.89607738
TTGTATC130400.013.80218239
Produced by FastQC (version 0.11.5)